Study on the Characteristics and Application Value of Nanocatalysts Prepared by Enzymatic Cleavage

DOI: https://doi.org/10.62517/jlsa.202507106

Author(s)

Qihariga1, Tegxibaiyin Wang2,*

Affiliation(s)

1Inner Mongolia Medical University, Hohhot, China 2Affiliated Hospital of Inner Mongolia Minzu University, Tongliao, China ✳Corresponding Author.

Abstract

Nano-enzyme is a kind of functional nanomaterial with enzyme-like catalytic activity. Among them, Peroxidase-like nanozymes have attracted much attention due to their great potential applications in biosensing. This article provides an overview of the various types of nanozyme reactions, the factors influencing their activity, and their applications across different fields. Additionally, it briefly discusses the characteristics of the enzymatic cleavage method for preparing nanozymes, offering a theoretical foundation for the future clinical applications of nanozymes.

Keywords

Nanozyme; Enzymatic Cleavage; Nanozyme Applications

References

[1]Manea F., Houillon F.B., Pasquato L., et al. Nanozymes:gold nanoparticle basedtransphosphorylationCatalysts.Angew.Chem.,Int.Ed.2004,43(45):6165-6169. [2]L Gao, J Zhuang, L Nie, J Zhang, Y Zhang, N Gu, T Wang, J Feng, D Yang, S Perrett and X Yan. Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nature nanotechnology, 2007, 2(9): 577-583. [3]Gao L Z, Liang M M, Wen T, et al. Research on Scientific and Technological Terms of China, 2020, 22(06): 21. [4]Wei H, Wang E K. Chem. Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes. Soc. Rev., 2013, 42(14): 6060 [5]Gao L Z, Chen L, Zhang R F, Yan X Y. Nanozymes: next-generation artificial enzymes. Scientia Sinica(Chimica). 2022, 52(9): 1649-1663. [6]Fan K L, Gao L Z, Wei H, et al. Nanozymes, Progress in Chemistry, 2023, 35(1):1-87LIN Y, REN J, QU X. Nano-gold as artificial enzymes: hidden talents. Adv Mater, 2014, 26(25): 4200-4217. [7]HE W, ZHOU Y T, WAMER W G, et al. Mechanisms of the pH dependent generation of hydroxyl radicals and oxygen induced by Ag nanoparticles. Biomaterials, 2012, 33(30): 7547-7555. [8]PEDONE D, MOGLIANETTI M, DE LUCA E, et al. Platinum nanoparticles in nanobiomedicine. Chem Soc Rev, 2017, 46(16): 4951-4975. [9]GE C, FANG G, SHEN X, et al. Facet energy versus enzyme-like activities: the unexpected protection of palladium nanocrystals against oxidative damage. ACS Nano, 2016, 10(11): 10436-10445. [10]XU D, WU L, YAO H, et al. Catalase-like nanozymes: classification, catalytic mechanisms, and their applications. Small, 2022, 18(37): e2203400. [11]CHEN Z, YIN J J, ZHOU Y T, et al. Dual enzyme-like activities of iron oxide nanoparticles and their implication for diminishing cytotoxicity. ACS Nano, 2012, 6(5): 4001-4012. [12]RAGG R, NATALIO F, TAHIR M N, et al. Molybdenum trioxide nanoparticles with intrinsic sulfite oxidase activity. ACS Nano, 2014, 8(5): 5182-5189. [13]CHEN M, WANG Z, SHU J, et al. Mimicking a natural enzyme system: cytochrome c oxidase-like activity of Cu2O nanoparticles by receiving electrons from cytochrome c. Inorg Chem, 2017, 56(16): 9400-9403. [14]LEE J, LIAO H, WANG Q, et al. Exploration of nanozymes in viral diagnosis and therapy. Exploration, 2022, 2(1): 20210086. [15]CASALS E, ZENG M, PARRA-ROBERT M, et al. Cerium oxide nanoparticles: advances in biodistribution, toxicity, and preclinical exploration. Small, 2020,16(20): e1907322. [16]CHEN T, LU Y, XIONG X, et al. Hydrolytic nanozymes: preparation, properties, and applications. Adv Colloid Interface Sci,2024,323:103072. [17]LEE J, LIAO H, WANG Q, et al. Exploration of nanozymes in viral diagnosis and therapy. Exploration, 2022,2(1):20210086. [18]Ahmed S, Ikram S. Biosynthesis of gold nanoparticles: a green approach. J Photochem, 2016, 161: 141-153. [19]Luo W, Zhu C, Su S, et al. Self-catalyzed, self-limiting growth of glucose oxidase-mimicking gold nanoparticles. ACS Nano, 2010, 4(12): 7451-7458. [20]Kalantari M, Ghosh T, Liu Y, et al. Highly thiolated dendritic mesoporous silica nanoparticles with high-content gold as nanozymes: the nano-gold size matters. ACS Appl Mater Interfaces, 2019, 11(14): 13264-13272. [21]Valden M, Lai X, Goodman D W. Onset of catalytic activity of gold clusters on titania with the appearance of nonmetallic properties. Science, 1998, 281(5383): 1647-1650. [22]Singh N, Savanur M A, Srivastava S, et al. A redox modulatory Mn3O 4 nanozyme with multi‐enzyme activity provides efficient cytoprotection to human cells in a Parkinson’s disease model. Angew Chem, 2017, 129(45): 14455-14459. [23]Li J, Xie C, Huang J, et al. Semiconducting polymer nanozymes with photothermic activity for enhanced cancer therapy. Angew Chem Int Ed Engl, 2018, 57(15): 3995-3998. [24]Fan K, Wang H, Xi J, et al. Optimization of Fe3O4 nanozyme activity via single amino acid modification mimicking an enzyme active site. Chem Comm (Camb), 2016, 53(2): 424-427. [25]Zhang X, Gao Y F. Colorimetric Detection of Hydrogen Peroxide Based on Molybdenum Disulfide Nanonzyme. Chinese J Anal Chem, 2022, 50(10):1491-1501 [26]Wang B, Zhang X D, Kang G, et al. Colorimetric Detection of Alkaline Phosphatase Activity Based on Manganese Single Atom Nanozyme. Chinese J Anal Chem, 2022, 50(1): 54-63. [27]Wang S, Cazelles R, Liao W C, et al. Mimicking horseradish peroxidase and NADH peroxidase by heterogeneous Cu2 + modified graphene oxide nanoparticles. Nano Lett, 2017, 17(3):2043-2048.