Preparation and Evaluation of Florfenicol Nanocrystals for in Vivo Intestinal Absorption

DOI: https://doi.org/10.62517/jmhs.202405116

Author(s)

Guanli Gong1,2, Huan Li1,2, Jiaoyan Luo1, Junfeng Ban1,2,*

Affiliation(s)

1School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong, China 2Pharmaceutical Innovation Integration Team, Guangzhou, Guangdong, China *Corresponding Author.

Abstract

Initiatives to avoid or reduce the use of antibiotics have a positive significance, and maximizing the retention of the effectiveness of existing antibiotics is one of the effective measures that can be taken in the field of food animal husbandry. A "top-down" method was used to prepare florfenicol nanocrystals, which appeared as irregular prismatic structures under electron microscopy. The particle size measured by laser diffraction was (291.9±2.9) nm, and the zeta potential value was - (23.63±1.64) mV. The absorption results in vivo showed that florfenicol was absorbed in the duodenum, jejunum, ileum, and colon, but primarily in the duodenum. The effective permeability coefficient (Peff) of florfenicol increased to 1.8 times that of the bulk drug group after being prepared as nanocrystals. The above research results indicate that nanocrystal technology has a promoting effect on the intestinal absorption of florfenicol.

Keywords

Florfenicol; Nanocrystals; In Vivo Evaluation; Intestinal Absorption

References

[1] Kanna D Y, Lam M K, Chai Y H, et al. Unlocking the potential of microalgae bio-factories for carbon dioxide mitigation: A comprehensive exploration of recent advances, key challenges, and energy-economic insights. Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies, 2023. [2] Bean-Hodgins L, Kiarie E G. Mandated restrictions on the use of medically important antibiotics in broiler chicken production in Canada: implications, emerging challenges, and opportunities for bolstering gastrointestinal function and health — a review. Canadian Science Publishing, 2021(4). [3] Qiu K, Li C L, Wang J, et al. Effects of Dietary Supplementation With Bacillus subtilis, as an Alternative to Antibiotics, on Growth Performance, Serum Immunity, and Intestinal Health in Broiler Chickens. Frontiers in Nutrition, 2021. [4] Seerengaraj V, Sun Y Z, Maruthupandy M, et al. Dietary Supplementation of Nanomaterials Additives in Aquaculture - A Review. International Journal of Pharmaceutical Sciences Review and Research, 2021. [5] Mehdi Y, Létourneau-Montminy M, Gaucher M L, et al. Use of antibiotics in broiler production:Global impacts and alternatives. Animal Nutrition, 2018. [6] Quik J T K, Meesters J A J, Peijnenburg W J G M, et al. Environmental Risk Assessment (ERA) of the application of nanoscience and nanotechnology in the food and feed chain. EFSA Supporting Publications, 2020, 17(11). [7] Roth N A S U. The application of antibiotics in broiler production and the resulting antibiotic resistance in Escherichia coli: A global overview. Poultry Science, 2019, 98(4). [8] Ogal M, Johnston S L, Klein P, et al. Echinacea reduces antibiotic usage in children through respiratory tract infection prevention: a randomized, blinded, controlled clinical trial. Eur J Med Res, 2021, 26(1):33. [9] Montanarella F, Kovalenko M V. Three Millennia of Nanocrystals. ACS Nano, 2022, 16(4):5085-5102. [10] Qiu J, Nguyen Q N, Lyu Z, et al. Bimetallic Janus Nanocrystals: Syntheses and Applications. Adv Mater, 2022, 34(1):e2102591. [11] Si Y, Luo H, Zhou F, et al. Advances in polysaccharide nanocrystals as pharmaceutical excipients. Carbohydr Polym, 2021, 262:117922.