The Effect of Total Glucosides of Peony on Lipopolysaccharide Induced Monocyte Immune Inflammation

DOI: https://doi.org/10.62517/jmhs.202405213

Author(s)

Sheng Li, Xia Wang

Affiliation(s)

College of Basic Medical Sciences, Jinggangshan University, Ji’an, Jiangxi, China

Abstract

This study aimed to explore the effect of total glucosides of peony (TGP) on the lipopolysaccharide induced secretion of IL-1, IL-6 and TNF-α in monocytes. Human peripheral blood was used to isolate and culture monocytes, with 25μg/mL Porphyromonas gingivalis lipopolysaccharide (Pg LPS) as a stimulating factor, then we observed the inhibit influence of 1, 5, 10, 20, 50μg/mL concentrations of TGP on the secretion of IL-1, IL-6, and TNF-α. Enzyme-linked immunosorbent assay (ELISA) was used on the concentration determination of IL-1, IL-6, and TNF-α. This study discovered that TGP had a significant inhibitory effect on Pg LPS induced monocyte secretion of IL-1 and IL-6, and the inhibitory effect on IL-1 was enhanced with the increase of TGP concentration. However, when the TGP reached a certain concentration, the inhibitory effect on IL-6 weakens. No effect was observed on the inhibitory effect of TNF-α. The results of the experiments showed that TGP have a significant inhibitory effect on the secretion of IL-1 and IL-6 by monocytes induced by Pg LPS within a certain concentration range, which might be one of its mechanisms in treating periodontitis.

Keywords

Total Glucosides of Paeony; Porphyromonas Gingivalis Lipopolysaccharide; Periodontitis; Traditional Chinese Medicine; Therapy

References

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