Study on Anti-Inflammatory Effects and Mechanism of Sojae Semen Praeparatum

DOI: https://doi.org/10.62517/jmhs.202405402

Author(s)

Siqing Wen, Yuting Xu, Linghao Qin, Xiaoming Wang*

Affiliation(s)

School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong, China *Corresponding Author.

Abstract

This study explores the anti-inflammatory effects and mechanisms of Sojae Semen Praeparatum (SSP) through network pharmacology and animal experimentation. First, active ingredients and therapeutic targets in SSP were identified using the TCMSP database and literature review. In addition, inflammation-related ingredients, targets, and pathways were sourced from databases such as TTD, KEGG, and Drug Bank. These findings were used to construct and analyze networks of ingredient-target-pathway interactions, target distribution across tissues, and key protein associations. Finally, a rat paw edema test was performed to experimentally confirm SSP's anti-inflammatory effects. The results identified 16 inflammation-related active ingredients in SSP, which act on 33 target proteins across 73 pathways. Furthermore, SSP extracts significantly reduced paw edema in rats, indicating strong anti-inflammatory potential. This study demonstrates that SSP exerts anti-inflammatory effects through multi-ingredient, multi-target, and multi-pathway interactions, providing a scientific basis for its clinical application.

Keywords

Sojae Semen Praeparatum; Anti-Inflammatory; Network Pharmacology; Mechanism of Action; Paw Edema

References

[1] Tao Hongjing, edited by Shang Zhijun. Ming Yi Bie Lu. Beijing: People's Medical Publishing House, 1986: 342. [2] Zhang Hong, Peng Huasheng. Verification of the Properties, Meridian Tropism, and Efficacy of Sojae Semen Praeparatum. Journal of Yunnan Traditional Chinese Medicine and Materia Medica, 2021, 42(12): 68-70. [3] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China (Volume I). Beijing: China Medical Science Press, 2020: 342. [4] He Yongyu. Study on the Anti-Influenza Virus Effect and Mechanism of Sojae Semen Praeparatum Decoction. Zhejiang Academy of Medical Sciences, 2023. [5] Qixin Yang, Linhua Tian, Shuyu Li, et al. Semen Sojae Praeparatum Improves Anxiety in Mice by Inhibiting HPA Axis Hyperactivity and Modulating Gut Microbiota. Journal of Functional Foods, 2022, 98: 105282. [6] The UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2023, Nucleic Acids Research, Volume 51, Issue D1, 6 January 2023, Pages D523-D531. [7] Kanehisa, M., Furumichi, M., Sato, Y., et al. KEGG for Taxonomy-Based Analysis of Pathways and Genomes. Nucleic Acids Res. 2023, 51: D587-D592. [8] Knox C, Wilson M, Klinger CM, et al. DrugBank 6.0: the DrugBank Knowledgebase for 2024. Nucleic Acids Res. 2024 Jan 5;52(D1): D1265-D1275. [9] Wang Y, Zhang S, Li F, et al. Therapeutic Target Database 2020: Enriched Resource for Facilitating Research and Early Development of Targeted Therapeutics. Nucleic Acids Res, 2019. [10] Szklarczyk D, Kirsch R, Koutrouli M, et al. The STRING Database in 2023: Protein-Protein Association Networks and Functional Enrichment Analyses for Any Sequenced Genome of Interest. Nucleic Acids Res. 2023, 51(D1): D638-D646. [11] Morita I, Schindler M, Regier K, et al. Different Intracellular Locations for Prostaglandin Endoperoxide H synthase-1and-2. J Biol Chem, 1995,270(18): 10902-10908. [12] Guo Qiuyan. Network Target Prediction and Mechanism Study of Guizhi Shaoyao Zhimu Decoction in Treating Rheumatoid Arthritis. Beijing: China Academy of Chinese Medical Sciences, 2016. [13] Wang Pingping, Kong Fanping, Chen Xuequn, et al. HIF-1 Signal Pathway in Cellular Response to Hypoxia. Journal of Zhejiang University (Medical Edition), 2011, 40(05): 559-566. [14] Chen Ying, Zhang Wenling, Huang Tao, et al. Relationship between Inflammatory Factors TNF-α, IL-6, IL-17 and Rheumatoid Arthritis Complicated with Atherosclerosis. Immunological Journal, 2017, 33(03): 268-272.