A Network Pharmacological Study of Chicory in the "Homotherapy for Heteropathy" of Uric Acid Metabolic Diseases
DOI: https://doi.org/10.62517/jmhs.202605301
Author(s)
Minghui Niu¹, Zhuang Xu2, Dingjie Luo2, Lanfeng Wei2, Xiaoyu Dong2, Shuai Chen2*, Xiaowen Wang3*
Affiliation(s)
1Pharmacy Intravenous Admixture Services (PIVAs) Shihezi People's Hospital, Shihezi, Xinjiang, China
2Xinjiang Key Laboratory of New Energy Materials and Green Chemical Engineering Xinjiang Institute of Engineering, Urumqi, Xinjiang, China;
3Xinjiang Zhengsheng Nutrition Research Co., Ltd., Changji, Xinjiang, China.
*Corresponding Author
Abstract
To decipher the intrinsic regulatory mechanism of chicory in the “homotherapy for heteropathy” of uric acid metabolic diseases using network pharmacology approaches. Methods: TCMSP, HERB, PubChem as well as Swiss Target Prediction, were adopted for the identification of potential compound targets screen the active ingredients and associated targets of chicory. Potential disease targets were acquired using GeneCards and OMIM. Venn diagrams were constructed to identify intersecting targets. The overlapping targets were uploaded to the STRING database for retrieval of protein–protein interaction (PPI) data and subsequent construction of PPI networks. Subsequently, Shared intersective targets were loaded into STRING to obtain comprehensive PPI information, which supported the generation of protein interaction networks. Functional annotation analyses relying on GO and KEGG databases were carried out to characterize the functional features and key pathways associated with screened targets.based on the overlapping targets. Finally, molecular docking and target tissue localization were conducted. Results: Overall 21 bioactive constituents of chicory were yielded, among which quercetin, luteolin, and kaempferol corresponded to relatively high numbers of targets. Sixty-seven intersecting targets between ingredients and diseases were collected. Key targets analyzed by component–target–pathway (C–T–P) and PPI networks were ESR1, TNF, IL6, CASP3, and IL1B. GO enrichment yielded 2353 functional items, and KEGG enrichment yielded 136 pathways. Molecular docking revealed high-binding combinations of key targets as follows: ESR1 with M14, TNF with M13, TNF with M14, IL6 with M13, CASP3 with M3, and IL1B with M1. Tissue localization showed that targets were mainly concentrated in the liver and intestine. Conclusions: As a traditional Chinese medicine, chicory exerts therapeutic effects on uric acid–related metabolic achieve therapeutic intervention on iseases via a synergistic mode featuring multiple phytochemicals and extensive target regulation, multipathway, and multiorgan characteristics.
Keywords
Chicory; Network Pharmacology; Homotherapy for Heteropathy; Uric Acid; Metabolic Diseases; Mechanism Study.
References
[1]Yang Z , Lyu B , Ma B , et al. Screening of the effective sites of Cichorium glandulosum against hyperuricemia combined with hyperliSSpidemia and its network pharmacology analysis.Computational Biology & Chemistry, 2024, 110(000).
[2]Street R A, Sidana J, Prinsloo G. Cichorium intybus: Traditional uses, phytochemistry, pharmacology, and toxicology. Evid-Based Complementary Altern Med, 2013, 2013(6):579319.
[3]Cheng-Yuan W, Jian-Gang D .Research progress on the prevention and treatment of hyperuricemia by medicinal and edible plants and its bioactive components. Frontiers in Nutrition, 2023, 10(000):14.
[4]Zhang GB, Li QY, Chen QL, et al. Network pharmacology: a new approach for chinese herbal medicine research. Evid Based Complement Alternat Med, 2013, 2013: 621423.
[5]Yao F, Zhang R, Fu R, et al. Preventive and therapeutic effects of quercetin on hyperuricemia and renal injury in rats. Wei sheng yan jiu Journal of hygiene research, 2011, 40(2):175.
[6]Luteolin ameliorates hyperuricemic nephropathy by activating urate excretion and Nrf2/HO-1/NQO1 antioxidant pathways in mice.Food science & nutrition, 12(10):8053-8066.
[7]Wang Y, Zhang G, Pan J, et al. Novel insights into the inhibitory mechanism of kaempferol on xanthine oxidase. J Agric Food Chem, 2015, 63(2): 526-34.
[8]Alshehri A S .Kaempferol attenuates diabetic nephropathy in streptozotocin-induced diabetic rats by a hypoglycaemic effect and concomitant activation of the Nrf-2/Ho-1/antioxidants axis. Archives of Physiology and Biochemistry, 2021(740671):1-14.
[9]Wang W, Liou TH, Lee WJ, et al. ESR1 gene and insulin resistance remission are associated with serum uric acid decline for severely obese patients undergoing bariatric surgery. Surg Obes Relat Dis. 2014, 10(1):14-22
[10]Zhou Y M, Zhao M, Pu Z, et al. Relationship between oxidative stress and inflammation in hyperuricemia: Analysis based on asymptomatic young patients with primary hyperuricemia. Medicine, 2018, 97(49):8.
[11]Jiang Z, Wang G, Meng L, et al. Protective Effects of Astragaloside IV on Uric Acid-Induced Pancreatic β-Cell Injury through PI3K/AKT Pathway Activation. Evidence-Based Complementary and Alternative Medicine, 2022, 2022:2429162.
[12]Wei C , Xi-Mei D , Ying L ,et al. Uric Acid Induces Endothelial Dysfunction by Activating the HMGB1/RAGE Signaling Pathway. BioMed Research International, 2017, 2017:4391920.
[13]Liu P, Xu H, Shi Y, et al. Potential molecular mechanisms of plantain in the treatment of gout and hyperuricemia based on network pharmacology. Evidence-Based Complementary and Alternative Medicine, 2020, 2020: 3023127.
[14]Yuan X, Li N, Zhang M, et al. Taxifolin attenuates IMQ-induced murine psoriasis-like dermatitis by regulating T helper cell
[15]Yue Z, Bing Z, Zhi-Jian L, et al. Virtual screening for components in Chicory combined with CNT2 target based on molecular docking. China Journal of Chinese Materia Medica, 2016, 41(21):3962.
[16]Nutmakul T. A review on benefits of quercetin in hyperuricemia and gouty arthritis. Saudi Pharm J, 2022, 3(7): 918-926.
[17]Hui L I, Liu X Q, Zhang B, et al. Effects of active component in Cichorii on lipid metabolism of rat with hypertriglyceridemia complicated by hyperuricemia and hyperglycemia. Journal of Integrative Medicine (JIM), 2008, 6(2):157
